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Prediction of centrifugation times for equilibrium and velocity sedimentation on various gradients

โœ Scribed by David B. Bylund; George Bruening


Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
538 KB
Volume
58
Category
Article
ISSN
0003-2697

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โœฆ Synopsis


Computer and calculator programs have been prepared which can predict sedimentation times for a significant fraction of the kinds of density gradient centrifugation experiments currently being carried out in biochemistry. Gradients of sucrose, glycerol, or C&l were accommodated for linear or several forms of convex or concave gradient profiles. Times of sedimentation to various uniformly spaced points between the meniscus and t,he bottom of the tube, or to a point near the isopycnic density, are predicted.


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A separation chamber having a surface of 50 cm\* and a height of 2 cm is described for the rapid separation of cells and cell organelles at acceleration forces from 10 to 90g. To eliminate wall sedimentation artifacts, the chamber was positioned 20 cm from the rotor axis in a speedcontrolled centrif