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Continuous production of NADP by immobilized Brevibacterium ammoniagenes cells

✍ Scribed by Kousaku Murata; Jyoji Kato; Ichiro Chibata

Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
324 KB
Volume
21
Category
Article
ISSN
0006-3592

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✦ Synopsis

Abstract

Whole cells of Brevibacterium ammoniagenes IAM 1645 having the polyphosphate NAD‐kinase were successfully immobilized in a polyacrylamide gel lattice. The immobilized cells were activated by treatment with organic solvents or detergents. The pH optimum of the immobilized cells for the production of NADP was 7.0, and divalent metal ions were required to maintain the elevated activity of polyphosphate NAD‐kinase. Highly pure NADP was continuously produced in high yield by the immobilized cell column. The half‐life of this column was about eight days.

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