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Continuous-Exchange Cell-Free Protein-Synthesizing System: Synthesis of HIV-1 Antigen Nef

✍ Scribed by Marina N. Chekulayeva; Oleg V. Kurnasov; Vladimir A. Shirokov; Alexander S. Spirin


Book ID
115587261
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
83 KB
Volume
280
Category
Article
ISSN
0006-291X

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Dihydrofolate reductase was synthesized in a batch system in the presence of the af6Nty ligand methotrexate, bound to various matrices. l k o types of gel were used: commercial methotrexate-agarose with pores inaccessible for translation machinery and methotrexate-POROS with pores easily accessible