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Continuous ethanol fermentation of lactose by a recombinant flocculating Saccharomyces cerevisiae strain

✍ Scribed by Lucília Domingues; Maria M. Dantas; Nelson Lima; José A. Teixeira

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
132 KB
Volume
64
Category
Article
ISSN
0006-3592

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✦ Synopsis

Alcohol fermentation of lactose was investigated using a recombinant flocculating Saccharomyces cerevisiae, expressing the LAC4 (coding for ␤-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces marxianus. Data on yeast fermentation and growth on a medium containing lactose as the sole carbon source are presented. In the range of studied lactose concentrations, total lactose consumption was observed with a conversion yield of ethanol close to the expected theoretical value. For the continuously operating bioreactor, an ethanol productivity of 11 g L -1 h -1 (corresponding to a feed lactose concentration of 50 g L -1 and a dilution rate of 0.55 h -1 ) was obtained, which is 7 times larger than the continuous conventional systems. The system stability was confirmed by keeping it in operation for 6 months.

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