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Continuous centrifugation in virus processing

✍ Scribed by Patrick, William C. ;Freeman, Robert R.

Publisher
Wiley (John Wiley & Sons)
Year
1960
Weight
402 KB
Volume
2
Category
Article
ISSN
0368-1467

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✦ Synopsis

Abstract

Ambler's Q/Ξ£ relationship between the throughput of a centrifuge and its mechanical characteristics has been translated from batch data in order to predict operating conditions for continuous centrifugation in virus processing. A Sharples Laboratory Supercentrifuge was converted from batch to continuous operation and a slurry feed device was developed. A correlation between operating conditions for continuous centrifugation and amount of test virus recovered from the feed showed that the Q/Ξ£ values at which high concentrations of the virus were recovered were almost identical to the Q/Ξ£ values for significant virus recovery in batch centrifugation. Titration and gravimetric determinations of the sediments recovered from continuous centrifugation operations indicate that product purity might be influenced by operating conditions.

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