β Scribed by Patrick, William C. ;Freeman, Robert R.
No coin nor oath required. For personal study only.
The mathematical relationship between the throughput of a batch centrifuge and its mechanical characteristics has been applied to the scaleβup of one of the steps in virus processing for possible application to the production of vaccines and similar antigens. Slurries that contained a test virus were purified in a laboratory centrifuge. The Q/Ξ£ relationship, developed by Ambler, was used to predict the operating conditions of a larger centrifuge in order to achieve the same clarification of slurry as that produced in the laboratory centrifuge. A correlation between conditions of centrifugation and amount of virus removed from the feed to the centrifuge shows that there is a wellβdefined Q/Ξ£ value at which significant concentrations of the test virus are sedimented.
π SIMILAR VOLUMES
## Abstract The mathematical theory of the sedimentation type of centrifuge is developed in terms of unhindered settling of a single particle that has reached equilibrium velocity. From this is derived the sigma value for several of the different forms of sedimentation type centrifuge which is the
## Abstract This case study focuses on the scaleβup of a Sp2/0 mouse myeloma cell line based fedβbatch bioreactor process, from the initial 3βL bench scale to the 2,500βL scale. A stepwise scaleβup strategy that involved several intermediate steps in increasing the bioreactor volume was adopted to