Continuous assay for DNA polymerization by light scattering
β Scribed by K.A. Johnson; F.R. Bryant; S.J. Benkovic
- Book ID
- 107712027
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 261 KB
- Volume
- 136
- Category
- Article
- ISSN
- 0003-2697
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A versatile continuous fluorometric assay for phospholipases A2, C, and D has been developed utilizing polymerized mixed liposomes made of pyrene-containing phospholipids (5 mol%) uniformly inserted in the polymerized liposomes of 1,2-bis[12-(lipoyloxy)dodecanoyl]-sn-glycero-3-phosphoglycerol (BLPG)
## Abstract Exposure of __Limulus__ amoebocyte lysate to endotoxin under stirring produced lightβreflective particles that appeared to be coagulin polymers. A laser lightβscattering particle counter, the PAβ200, detected these particles sensitively. The PAβ200 detected endotoxin at a concentration