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Construction of viable mouse-human hybrid cells by nuclear transplantation

✍ Scribed by Margaret J. Hightower; Joseph J. Lucas

Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
986 KB
Volume
105
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

Viable interspecies cytoplasmic‐nuclear hybrid cells were constructed by fusion of karyoplasts prepared from the highly tumorigenic A9 mouse fibroblast cell line and cytoplasts prepared from the Detroit 532 normal human diploid cell strain. The identity of the hybrid cells was ascertained using a variety of morphological, immunological, and genetic criteria, including: nuclear pattern of staining with the fluorochrome Hoechst 33258, appearance of the actin‐myosin containing cytoskeleton, presence of fibronectin, and resistance to azaguanine and diphtheria toxin. About 90% of the hybrid cells were viable, that is, capable of division. Changes in the morphology of the hybrid cells, apparently nuclear directed, were observed before cell division occurred. Using the techniques described here, large numbers of interspecies hybrid cells suitable for many types of biochemical analyses can be routinely produced.

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