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Construction of Templates for Sequencing Extended DNA Fragments

✍ Scribed by M. A. Ermolaeva; A. V. Soldatov; E. N. Nabirochkina


Book ID
110410271
Publisher
SP MAIK Nauka/Interperiodica
Year
2002
Tongue
English
Weight
209 KB
Volume
36
Category
Article
ISSN
0026-8933

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In an effort to find a simple and inexpensive purification method of polymerase chain reaction (PCR) reaction before cycle sequencing reaction, we compared a commercial system with a precipitation protocol performed in our laboratory. We found that, particularly with small PCR products, our method w