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Construction of plasmid vectors that facilitate subcloning and recovery of yeast and Escherichia coli DNA fragments

✍ Scribed by Naumovski, Louie; Friedberg, Errol C.


Book ID
123276274
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
661 KB
Volume
22
Category
Article
ISSN
0378-1119

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πŸ“œ SIMILAR VOLUMES


Construction and characterization of a p
✍ Little, John W. πŸ“‚ Article πŸ“… 1979 πŸ› Springer 🌐 English βš– 1002 KB

The E. coli recA gene was cloned from the phage lambda precA into the vector pBR313. A plasmid, pJL3, was also isolated by cloning a portion of the recA gene into the vector pBR322. pJL3 coded for a fragment of the recA protein 34 Kd (kilodaltons) in size (compared to 40 Kd for the intact protein).