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Construction of leaky strains and extracellular production of exogenous proteins in recombinant E scherichia coli

✍ Scribed by Chen, Zhao-Yuan; Cao, Jie; Xie, Li; Li, Xiao-Fei; Yu, Zhen-Hai; Tong, Wang-Yu

Book ID: 121840397
Publisher: Wiley (Blackwell Publishing)
Year: 2014
Tongue: English
Weight: 408 KB
Volume: 7
Category: Article
ISSN: 1751-7907
DOI: 10.1111/1751-7915.12127

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## Abstract Extracellular production of recombinant proteins in __Escherichia coli__ has several advantages over cytoplasmic or periplasmic production. However, nonpathogenic laboratory strains of __E. coli__ generally excrete only trace amounts of proteins into the culture medium under normal grow