Flow Cytometric Analysis of Metabolic St
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Elena Soriano; Nicole Borth; Hermann Katinger; Diethard Mattanovich
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Article
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1999
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Elsevier Science
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English
β 427 KB
Overexpression of recombinant proteins in Escherichia coli often leads to a severe growth retardation of the host cells. Using flow cytometry, we analyzed the temporal development of the cellular content of DNA, total protein, and the recombinant product (human superoxide dismutase) in different str