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Construction of integrated system for selection of fused plant protoplasts

โœ Scribed by Kazuo Sakamoto; Kazuhiro Fujita; Kimiko Hirayae; Kumiko Iida; Takashi Koyano; Yoshihisa Asada; Tsutomu Furuya

Book ID
104644720
Publisher
Springer-Verlag
Year
1993
Tongue
English
Weight
748 KB
Volume
7
Category
Article
ISSN
0951-208X

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โœฆ Synopsis

An integrated system has been constructed to instantly identify and efficiently sort the hcterokaryons formed by plant protoplast fusion. The system is composed of the following functions: a) a transport system, b) an electro-manipulator, c) a cell harvester, d) a flow cytometer/cell sorter, and e) a control device. The conditions for an efficient and reproducible enrichment of the heterokaryons have been investigated by this system using the fluorescein isothiccyanate stained protoplasts preparing from Glycyrrhiza &bra cell cultures and unstained protoplasts of Abrusprecatorius cell cultures which contain a large quantity of chlorophyll.

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