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Selection methods in bacilli for recombinants and transformants of intra- and interspecific fused protoplasts

✍ Scribed by T. Akamatsu; J. Sekiguchi

Publisher: Springer
Year: 1983
Tongue: English
Weight: 616 KB
Volume: 134
Category: Article
ISSN: 0302-8933
DOI: 10.1007/bf00407807

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✦ Synopsis

The intraspecific fusion frequencies obtained with the direct selection method on a semi-synthetic regeneration medium between strains of B. subtilis and B. licheniformis were distributed from 9.9 x 10-2 to 4.5 x 10-a, which was one or two orders higher than those of interspecific recombinations between B. subtilis and B. licheniformis.

The regeneration media were also useful for selecting interspecific transformants from plasmid carrier to noncarrier. This selection could be used as a primary selection method for inter-and intraspecific recombinants obtained by protoplast fusion.

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