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Construction of frameshift mutation hot spots within the tetracycline resistance gene of pBR322

โœ Scribed by Burnouf, Dominique; Fuchs, Robert P.P.


Book ID
122626726
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
370 KB
Volume
67
Category
Article
ISSN
0300-9084

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A novel forward mutational system, based on the acquisition of an Iq-d dominant phenotype from an initial Iq- recessive state, was used to identify second-site frameshift mutation [+/- 1(+/- 3n) events] within the N-terminal region of the lacI gene of Escherichia coli. The DNA sequences are describe