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Construction of chimaeric processed immunoglobulin genes containing mouse variable and human constant region sequences

✍ Scribed by Takeda, Shun-ichi; Naito, Takayuki; Hama, Kazuaki; Noma, Takafumi; Honjo, Tasuku


Book ID
109738650
Publisher
Nature Publishing Group
Year
1985
Tongue
English
Weight
410 KB
Volume
314
Category
Article
ISSN
0028-0836

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Materials and methods ### 2.1 Immunological reagents and buffers Anti-C1 q was supplied by Dako (Copenhagen, Denmark). All other antibodies and conjugates were made in our laboratory. Isotonic Verona1 buffer of pH 7.3 was prepared with 1 mM MgCI2 and 1 mM CaCI2.