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Construction of cDNA libraries from limiting amounts of material

โœ Scribed by John R McCarrey; Steven A Williams

Book ID
108476196
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
657 KB
Volume
5
Category
Article
ISSN
0958-1669

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cDNA library construction from small amo
cDNA library construction from small amounts of unfractionated RNA: Association of cDNA synthesis with polymerase chain reaction amplification
โœ Christine Domec; Bertrand Garbay; Michel Fournier; Jacques Bonnet ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 942 KB

We describe here a general and simple procedure for cDNA library construction making use of in vitro amplification of cDNA by polymerase chain reaction (PCR). The first-strand cDNA is synthesized from total RNA with a primer EcoRI-(dT)17 and oligo(dG) tailed. An oligonucleotide, EcoRI-BamHI-(dC)13,