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Construction of a pilot human YAC library in a recombination-defective yeast strain

✍ Scribed by Giuseppe Palmieri; Maria Giuseppina Miano; Amelia Casamassimi; Arturo Lania; Kyoko Kohno; David Schlessinger; Michele D'Urso; Terence Featherstone

Book ID
117522135
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
107 KB
Volume
188
Category
Article
ISSN
0378-1119

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We have constructed a bovine yeast artificial chromosome (YAC) library to provide a common resource for bovine genome research. We used leukocytes of a Japanese black bull (Bos taurus) as the DNA source, AB1380 for the yeast host, and pYAC4 for the vector. The library consists of 24,576 clones arran