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Construction of a mouse yeast artificial chromosome library in a recombination–deficient strain of yeast

✍ Scribed by Chartier, F. L.; Keer, J. T.; Sutcliffe, M. J.; Henriques, D. A.; Mileham, P.; Brown, S. D. M.

Book ID
109915916
Publisher
Nature Publishing Group
Year
1992
Tongue
English
Weight
825 KB
Volume
1
Category
Article
ISSN
1061-4036

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We have constructed a bovine yeast artificial chromosome (YAC) library to provide a common resource for bovine genome research. We used leukocytes of a Japanese black bull (Bos taurus) as the DNA source, AB1380 for the yeast host, and pYAC4 for the vector. The library consists of 24,576 clones arran