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Construction of a chimeric aminopeptidase by a combination of gene shuffling and mutagenesis

✍ Scribed by Satoru Nirasawa; Kiyoshi Hayashi


Book ID
106364055
Publisher
Springer Netherlands
Year
2007
Tongue
English
Weight
313 KB
Volume
30
Category
Article
ISSN
0141-5492

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Bacterial luciferases are heteropolymetric enzymes consisting of two non-identical subunits (alpha and beta). The t w o polypeptides are produced by transcription in the same direction of t w o genes, luxA and luxB, located immediately adjacent t o each other and separated by only 29 base pairs in t