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Construction and properties of a chimeric bacteriophage lysis gene

✍ Scribed by Robin E. Harkness; Werner Lubitz

Book ID
109314044
Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
353 KB
Volume
48
Category
Article
ISSN
0378-1097

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The EcoRI digestion products of phage T4 DNA have been examined using a phage DNA transformation assay. A 2.6 X 10(6) Dalton fragment was found to contain the rII genes. This fragment was purified and then treated with HindIII endonuclease. The cleavage products were ligated to the vector plasmid pB