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Construction and expression of a hybrid plasmid containing the Escherichia coli thrA and thrB genes

✍ Scribed by Cossart, Pascale ;Katinka, Michaël ;Yaniv, Moshe ;Girons, Isabelle Saint ;Cohen, Georges N.

Publisher
Springer
Year
1979
Tongue
English
Weight
591 KB
Volume
175
Category
Article
ISSN
0026-8925

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✦ Synopsis

In vitro recombination techniques were used to clone the Escherichia coli thrA and thrB structural genes in the plasmid vector pBR322. The chimeric plasmid was analyzed and characterized genetically, by restriction mapping and DNA sequencing. The limited expression of the threonine biosynthetic enzymes in the strain carrying the recombinant plasmid is discussed.

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