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Construction and Expression of a Eukaryotic Expression Vector Containing a Fusion Gene of the Hantaan Virus S Gene and Hsp70 Gene

✍ Scribed by Juan Gao; Bicheng Zhang; Shoujing Yang; Kainan Li; Hualin Xu; Yimin Xiong; Yiming Wang; Yan Wang; Weitian Xu; Yi Cheng; Guorong Zheng


Publisher
Springer
Year
2008
Tongue
English
Weight
217 KB
Volume
58
Category
Article
ISSN
0343-8651

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In vitro recombination techniques were used to clone the Escherichia coli thrA and thrB structural genes in the plasmid vector pBR322. The chimeric plasmid was analyzed and characterized genetically, by restriction mapping and DNA sequencing. The limited expression of the threonine biosynthetic enzy