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Construction and characterization of an absolute deletion mutant of Escherichia coli ribonuclease II

✍ Scribed by João Piedade; Rita Zilhão; Cecília Maria Arraiano

Book ID
109320524
Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
728 KB
Volume
127
Category
Article
ISSN
0378-1097

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A method was developed for the isolation of spontaneous mutants of Escherichia coli K-12 with deletions extending from the srl operon to the adjacent recA gene. The srl-recA deletion mutants were extremely sensitive to dNA-damaging agents; unable to support growth of the feckless red gam mutant lamb