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Construction and characterization of a sheep BAC library of three genome equivalents

✍ Scribed by Daniel Vaiman, Alain Billault, Kamila Tabet-Aoul, Laurent Schibler, Didier Vilette, Anne Oustry-Vaiman, Catherine Soravito, Edmond P. Cribiu

Book ID
113034909
Publisher
Springer-Verlag
Year
1999
Tongue
English
Weight
267 KB
Volume
10
Category
Article
ISSN
0938-8990

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Construction and characterization of a gridded cattle BAC library
✍ J Buitkamp; S Kollers; G Durstewitz; R Fries; K Welzel; K Schäfer; A Kellermann; 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 137 KB

A bovine genomic large‐insert bacterial artificial chromosome (BAC) library has been constructed from leukocytes of a Holstein‐Friesian male. Size fractionated __Dpn__II‐digested genomic DNA was ligated to the dephosphorylated __Bam__H1 ends of a pBACe3·6 vector. Approximately 8·3×104 individual BAC