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Construction and Characterization of a Human Chromosome 2-Specific BAC Library

✍ Scribed by Min Wang; Xiao-Ning Chen; Stephanie Shouse; Jim Manson; Qingzhou Wu; Rumei Li; Janet Wrestler; David Noya; Zhi-Guang Sun; Julie Korenberg; Eric Lai


Book ID
115612508
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
598 KB
Volume
24
Category
Article
ISSN
0888-7543

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A bovine genomic large‐insert bacterial artificial chromosome (BAC) library has been constructed from leukocytes of a Holstein‐Friesian male. Size fractionated __Dpn__II‐digested genomic DNA was ligated to the dephosphorylated __Bam__H1 ends of a pBACe3Β·6 vector. Approximately 8Β·3Γ—104 individual BAC