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Construction and characterization of a fosmid library for pathogenic bacteriumVibrio anguillarum

✍ Scribed by Yifan Han; Zhaolan Mo; Yunxiang Mao; Peng Xiao; Jie Li; Bin Hao; Dongsheng Guo


Book ID
106031953
Publisher
Springer
Year
2009
Tongue
English
Weight
631 KB
Volume
27
Category
Article
ISSN
0254-4059

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A bovine genomic large‐insert bacterial artificial chromosome (BAC) library has been constructed from leukocytes of a Holstein‐Friesian male. Size fractionated __Dpn__II‐digested genomic DNA was ligated to the dephosphorylated __Bam__H1 ends of a pBACe3Β·6 vector. Approximately 8Β·3Γ—104 individual BAC