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Construction and characterization of a BAC library from a gynogenetic channel catfishIctalurus punctatus

✍ Scribed by Sylvie MA Quiniou; Takayuki Katagiri; Norman W Miller; Melanie Wilson; William R Wolters; Geoffrey C Waldbieser

Book ID
114998161
Publisher
BioMed Central
Year
2003
Tongue
French
Weight
461 KB
Volume
35
Category
Article
ISSN
0999-193X

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Construction and characterization of a g
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A bovine genomic large‐insert bacterial artificial chromosome (BAC) library has been constructed from leukocytes of a Holstein‐Friesian male. Size fractionated __Dpn__II‐digested genomic DNA was ligated to the dephosphorylated __Bam__H1 ends of a pBACe3Β·6 vector. Approximately 8Β·3Γ—104 individual BAC