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Constitutive homologous recombination between mitochondrial DNA and a linear mitochondrial plasmid inPhysarum polycephalum

โœ Scribed by Hiroyoshi Takano; Shigeyuki Kawano; Tsyneyoshi Kuroiwa

Publisher
Springer-Verlag
Year
1992
Tongue
English
Weight
863 KB
Volume
22
Category
Article
ISSN
0172-8083

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โœฆ Synopsis

In one particular myxamoebal strain (NG7; mF+) of Physarum polycephalum, a linear mitochondrial plasmid (mF plasmid) which promotes mitochondrial fusion has been identified. A mating between mF- strains, that do not carry the mF plasmid, resulted in uniparental inheritance of the mtDNA. In matings between mF+ and mF- strains a recombination occurred between the mtDNA and the mF plasmid, and recombinant mtDNA was generated with the end of the mF plasmid as its ends. The DNA sequences of the recombination site in the mtDNA and the mF plasmid, and of the recombinant mtDNA, revealed that the mF plasmid had a 473-bp sequence that was identical to, but slightly shorter than, a 477-bp sequence of the mtDNA. This so-called identical sequence was found at the junction between unique sequences of the mF plasmid and the mtDNA in the recombinant mtDNA. Thus, the recombination between the mtDNA and the mF plasmid was due to reciprocal crossing-over at the identical sequence.

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