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Conserved structure and organization of B hordein genes in theHor 2locus of barley

โœ Scribed by J. Vicente-Carbajosa; D. R. Beritashvili; A. S. Kraev; K. G. Skryabin

Publisher
Springer
Year
1992
Tongue
English
Weight
624 KB
Volume
18
Category
Article
ISSN
0167-4412

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โœฆ Synopsis

This work presents new information on the structure and organization of B hordein genes in the Hor 2 locus of barley. Data obtained by Southern blot analysis and cloning and sequencing of different members of this multigene family are discussed. B hordeins are the major group of seed storage proteins that accumulate in the barley endosperm. Genetic studies and extensive characterization at the protein level showed that they are encoded by a multigene family tightly linked at the Hor 2 locus.

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## Abstract mRNA species encoding B hordeins and B hordein protein have been localised in developing barley caryopses using in situ hybridisation and immunocytochemistry. B hordeins are first detected immunocytochemically in sections from 5 dpa (days post anthesis) barley caryopses. At this stage t