Chromatographic resolution of the subuni
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RennΓ©Chen Lu; Marshall Elzinga
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Article
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1977
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Elsevier Science
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English
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Two procedures are described for column chromatographic separation of the (I! and p subunits of brain tubulin using hydroxylapatite in the presence of(i) urea or (ii) sodium dodecyl sulfate and /3-mercaptoethanol. In the first system the a and /3 chains are partially resolved, and in the second the