Chromatographic resolution of the subunits of calf brain tubulin

✍ Scribed by RennéChen Lu; Marshall Elzinga

• Book ID: 102983998
• Publisher: Elsevier Science
• Year: 1977
• Tongue: English
• Weight: 963 KB
• Volume: 77
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(77)90310-4

✦ Synopsis

Two procedures are described for column chromatographic separation of the (I! and p subunits of brain tubulin using hydroxylapatite in the presence of(i) urea or (ii) sodium dodecyl sulfate and /3-mercaptoethanol. In the first system the a and /3 chains are partially resolved, and in the second the subunits are resolved into three peaks which we designate a,, (Ye. and p.

METHODS AND RESULTS

Preparation of Tub&in

Tubulin was prepared by the method of Weisenberg et al. ( 8) with some variations. Fresh calf brains were obtained from a local slaughterhouse. The cortexes of the brains were cleaned and, in a typical experiment, 800 g were homogenized in 800 ml of buffer (10 mM sodium phos-243 Copyright