## Abstract Conformation behavior of polyethylenimine has been examined by studies of the fluorescence characteristics of derivatives of the polymer containing pyrenyl ligands. Excimer formation within the macromolecular matrix serves as a sensitive probe of group proximities. The experimental obse
Conformational peculiarities of rRNAinffsupMetfromE. colias revealed by fluorescent methods
โ Scribed by A. N. Surovaya; O. F. Borissova
- Publisher
- Springer
- Year
- 1976
- Tongue
- English
- Weight
- 335 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0301-4851
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โฆ Synopsis
Conformational transitions in several individual tRNAs (tRNAMetf, tRNAPhe from E. coli, tRNAVal1, tRNASer, tRNAPhe from yeast) have been studied under various environmental conditions. The binding isotherms studies for dyes-tRNA complexes exhibited similarities in conformational states of all tRNAs investigated at low ionic strength (0.01 M NaCl). By contrast, at high ionic strength (0.4 M NaCl or 2 X 10(-4) M Mg2+) a marked difference is found in structural features of tRNAMetf as compared with other tRNAs used. The tRNAMetf is the only tRNA species that does not reveal the strong type of complexes with ethidium bromide, acriflavine and acridine orange.
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