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Conformational changes of Loxosceles venom sphingomyelinases monitored by circular dichroism

✍ Scribed by Sonia A. de Andrade; Matheus F. Fernandes Pedrosa; Rute M. Gonçalves de Andrade; Maria Luiza Vilela Oliva; Carmen W. van den Berg; Denise V. Tambourgi

Book ID: 116289672
Publisher: Elsevier Science
Year: 2005
Tongue: English
Weight: 289 KB
Volume: 327
Category: Article
ISSN: 0006-291X
DOI: 10.1016/j.bbrc.2004.11.146

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## Abstract The intensities of the CD bands at about 275 and 190 nm were monitored for DNAs with different G + C contents as a function of temperature. The 190‐nm bands showed a nearly complete and cooperative collapse on melting of the DNA, demonstrating that the CD arises from base–base interacti