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Influence of enzyme conformational changes on catalytic activity investigated by circular dichroism spectroscopy

✍ Scribed by Carolina Fortes Rigos; Hérica de Lima Santos; Geraldo Thedei Jr; Richard John Ward; Pietro Ciancaglini


Publisher
The American Society for Biochemistry and Molecular Biology
Year
2003
Tongue
English
Weight
87 KB
Volume
31
Category
Article
ISSN
1470-8175

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## Abstract The intensities of the CD bands at about 275 and 190 nm were monitored for DNAs with different G + C contents as a function of temperature. The 190‐nm bands showed a nearly complete and cooperative collapse on melting of the DNA, demonstrating that the CD arises from base–base interacti