Conformational and binding properties of chicken liver basic fatty acid binding protein in solution
โ Scribed by Elisabetta Schievano; Daniela Quarzago; Paola Spadon; Hugo Luis Monaco; Giuseppe Zanotti; Evaristo Peggion
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1994
- Tongue
- English
- Weight
- 701 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0006-3525
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Equilibrium constants for the binding of protoporphyrin to serum albumin and hemopexin and liver cytosolic fatty acid-binding protein of the rat were determined fluorometrically. The experimental equilibrium constant [10(6) M-1 (mean +/- S.D.)] values were 8.4 +/- 1.3, 10.0 +/- 2.4 and 34.0 +/- 3.0
A conformational study in solution of the fatty acid binding protein from chicken liver is presented. The nearly complete sequence-specific 1 H resonance assignment was achieved from homonuclear two-dimensional nmr experiments using a sample of native protein. The principal elements of secondary str
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## Abstract Former studies have linked hepatocyte growth with liver fatty acid binding protein (LโFABP) of rat liver cytosol. In search for the roles of LโFABP in hepatocytes, we previously stably transfected rat LโFABP sense and antisense cDNAs into rat hepatoma HTC cells that do not contain LโFAB