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Conductimetric assay of pyroglutamyl peptidase activity

✍ Scribed by Christine Besson; Sandrine Vessillier; Thierry Gonzales; Joëlle Saulnier; Jean Wallach

Book ID
102623008
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
387 KB
Volume
294
Category
Article
ISSN
0003-2670

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✦ Synopsis

A conductimetric method was applied to assay Streptococcus pyogenes pyroglutamyl peptidase (E.C. 3.4.8.11) activity using pyroglutamylalanine or pyroglutamyltyrosine. A spectrophotometric comparison using pyroglutamyl-/3naphthylamide indicated that the sensitivity was much increased when using conductimetry. The use of a C-protected dipeptide such as pyroglutamylalanineamide also increased the sensitivity. Kinetic parameters have been measured for both dipcptides, with K, values of 0.34 mM and 0.47 mM, respectively, for the alanine and tyrosine derivatives.

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