A fluorimetric assay for the indirect determination of superoxide production during the respiratory burst of stimulated polymorphonuclear leukocytes was described. The method allowed the determination of submicromolar concentrations of superoxide, and was sufficiently sensitive that first-derivative
The Development of Two Fluorimetric Assays for the Determination of Pyroglutamyl Aminopeptidase Type-II Activity
✍ Scribed by Seán P. Gallagher; Rhona M. O'Leary; Brendan O'Connor
- Book ID
- 102566467
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 167 KB
- Volume
- 250
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
Carnitine palmitoyltransferase II (CPT-II) mediates the import of long-chain fatty acids into the mitochondrial matrix for subsequent -oxidation. Defects of CPT-II manifest as a severe neonatal hepatocardiomuscular form or as a mild muscular phenotype in early infancy or adolescence. CPT-II deficie
A capillary electrophoresis assay of sulfoesterase activity was developed that overcomes the main drawbacks encountered with the usual methods for sulfate determination in complex biological medium. Conditions are described allowing direct measurement of inorganic sulfate that is enzymatically produ
## Abstract A new type of fluorescence assay for the determination of peroxidase (POx) activity is presented. The assay is based on the indication of the enzymatic consumption of H~2~O~2~ (HP), using a fluorescent europium–tetracycline (Eu~3~TC) complex as indicator. On addition of HP, this complex