Conditioned medium from activated splenocytes increases substance P in sympathetic ganglia

✍ Scribed by G. Miller Jonakait; S. Schotland

• Book ID: 102910804
• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 675 KB
• Volume: 26
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/jnr.490260104

✦ Synopsis

We report here that SP increases dramatically in cultures of SCG grown in the presence of conditioned medium from con-A-stimulated splenocytes. The effect is mimicked by growing SCG explants in the presence of human recombinant interleukin-1 (hrIL-1) but not hrIL-2. Nerve growth factor (NGF) is not involved in mediating this effect since antibodies to NGF included in the culture fail to alter the lymphokine-induced increase in SP. Moreover, the effect is somewhat specific for SP since the activities of tyrosine hydroxylase, tryptophan hydroxylase, and choline acetyltransferase (enzymes in the biosynthetic pathways for norepinephrine, serotonin, and acetylcholine) are not similarly elevated. Dorsal root ganglia respond with only modest increases in SP. The action of lymphokines in stimulating SP may, therefore, be a ganglion-specific action in promoting recovery following injury.