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Conditional knockout mouse for tissue-specific disruption of the cyclooxygenase-2 (Cox-2) gene

✍ Scribed by Tomo-o Ishikawa; Harvey R. Herschman

Publisher: John Wiley and Sons
Year: 2006
Tongue: English
Weight: 592 KB
Volume: 44
Category: Article
ISSN: 1526-954X
DOI: 10.1002/gene.20192

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✦ Synopsis

Cyclooxygenase-2 (Cox-2) modulates many normal functions, and appears to play a role in a wide variety of pathophysiologic conditions. Cox-2 gene expression is induced in many different cell types, in response to many distinct stimuli. We generated a conditional knockout mouse in which critical exons of the Cox-2 gene are flanked with loxP sites. Cox-2 flox/flox mice appear normal and are fertile. Recombination at the loxP sites, loss of Cox-2 protein expression, and prevention of induced PGE 2 accumulation are observed in Cox-2 flox/flox mouse embryo fibroblasts following infection with an adenovirus expressing CRE recombinase. In vivo recombination at the Cox-2 flox allele was demonstrated in the liver of Cox-2 flox/flox mice following intravenous injection of adenovirus expressing CRE recombinase. Spatially and temporally restricted elimination of the Cox-2 gene in Cox-2 flox/flox conditional knockout mice should provide a valuable tool to analyze the cell type-specific role of Cox-2 in many disease models. genesis 44: 143-149, 2006. V

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