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Condensation nucleation light scattering detection with microbore liquid chromatography for lipid analysis

✍ Scribed by Xiaohui Yang; John A. Koropchak

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
405 KB
Volume
12
Category
Article
ISSN
1040-7685

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✦ Synopsis

Methods of analysis for both neutral and phospholipids based on microbore high-pressure liquid chromatography HPLC coupled with detection by condensation nucleation light scattering detection are described. The limits of detection for phospholipids were below 100 ngrmL, corresponding to less than 0.5 ng or less than 1 pmole, but were even lower for neutral lipids, and down to Ž . 12 ngrmL 0.06 ng, 0.074 pmole . The method was applied to the determination of lipids in egg yolk samples.

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