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Comprehensive two-dimensional liquid chromatography with evaporative light-scattering detection for the analysis of triacylglycerols in Borago officinalis

✍ Scribed by Luigi Mondello; Marco Beccaria; Paola Donato; Francesco Cacciola; Giovanni Dugo; Paola Dugo

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
118 KB
Volume
34
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

An optimized 2‐D liquid chromatography (LC×LC) set‐up, based on the different selectivities of a silver ion (Ag) and a non‐aqueous reversed phase (NARP), employed in the first (D1) and the second dimension (D2), respectively, in combination with evaporative light‐scattering detection (ELSD), has been developed for the analysis of the triacylglycerol (TAG) fraction in a Borago officinalis oil. The 2‐D set‐up, thanks to the complementary separation selectivity provided by the two columns, allowed to distribute 78 TAGs throughout the 2‐D LC retention plane otherwise unachievable by 1‐D LC.

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## Abstract Microcolumn chromatography (1.0 mm to 0.3 mm column i.d.) of underivatized amino acids coupled to evaporative light‐scattering (ELS) detection demonstrated good baseline stability using perfluorinated carboxylic acid ion‐pairing agents of varying alkyl chain lengths (C~2~–C~8~) and conc