Computer assisted karyotype analysis ofPyrrhopappus carolinianus

✍ Scribed by J. L. Oud; F. Schuring

• Publisher: Springer Netherlands
• Year: 1987
• Tongue: English
• Weight: 916 KB
• Volume: 74
• Category: Article
• ISSN: 0016-6707
• DOI: 10.1007/bf00056116

✦ Synopsis

With the help of Computer Aided Karyotyping procedures, Ag-NOR staining and C-banding techniques, the karyotype of Pyrrhopappus caro#nianus (Asteraceae, Lactuceae) has been studied. The species has 2n = 12 chromosomes. Silver staining reveals that the two shortest pairs of chromosomes possess NOR's. On the basis of chromosome length and centromere position, only the longest chromosome pair and the satellite chromosomes can be identified. Two types of C-banding can be obtained, dependent on the temperature of the hydrochloric acid hydrolysis of the root tips. Hydrolysis at 60°C results exclusively in centromeric bands, whereas a treatment at room temperature reveals a pattern of intercalary bands. A computer assisted analysis of the intercalary banding pattern resulted in the construction of schematic representation of the average Cbanding pattern. This banding pattern allows an easy identification of each of the chromosome pairs.