The purpose of this study was to demonstrate the utility of combining a design of experiment (DOE) approach with high-throughput formulation screening to identify the main factors affecting protein thermostability and solution viscosity. The optimization of buffer compositions was guided by statisti
Compositional analysis and viscosity measurements of commercial oat brans
✍ Scribed by Luhaloo, M; Mårtensson, A-C; Andersson, R; Åman, P
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 312 KB
- Volume
- 76
- Category
- Article
- ISSN
- 0022-5142
No coin nor oath required. For personal study only.
✦ Synopsis
Chemical analysis of commercial oat brans (n \ 10) collected from Ðve di †erent countries showed wide variations in the contents of starch (40È61%), crude protein (9É6È21%), crude fat (8É2È12%), ash (2É0È4É1%), dietary Ðbre (10É7È 19É4%) and mixed-linkage b-glucan (4É7È8É3%). A signiÐcant correlation (r \ 0É83 ; P \ 0É005) between the contents of total and extractable mixed-linkage b-glucan was found. Reproducible increases in viscosity were noted for all samples when the Ðnely ground samples were dispersed in phosphate bu †er and viscosity development was followed for 100 min. Addition of mixed-linkage bglucanase greatly reduced the viscosity. Addition of amyloglucosidase, with no detectable mixed-linkage b-glucanase activity, increased viscosity, which could be related to an increased solubilisation of mixed-linkage b-glucan. Highly signiÐcant correlations (r [ 0É90) were found between the contents of both total and extractable mixed-linkage b-glucan and viscosity after dispersion.
1998 SCI.
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