The purpose of this study was to demonstrate the utility of combining a design of experiment (DOE) approach with high-throughput formulation screening to identify the main factors affecting protein thermostability and solution viscosity. The optimization of buffer compositions was guided by statisti
โฆ LIBER โฆ
Screening of monoclonal antibody formulations based on high-throughput thermostability and viscosity measurements: Design of experiment and statistical analysis
โ Scribed by He, Feng (author);Woods, Christopher E. (author);Trilisky, Egor (author);Bower, Keith M. (author);Litowski, Jennifer R. (author);Kerwin, Bruce A. (author);Becker, Gerald W. (author);Narhi, Linda O. (author);Razinkov, Vladimir I. (author)
- Publisher
- John Wiley and Sons Inc.
- Year
- 2011
- Tongue
- English
- Weight
- 418 KB
- Volume
- 100
- Category
- Article
- ISSN
- 0022-3549
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Differential scanning fluorimetry (DSF) was employed to increase the throughput of the thermostability screening of monoclonal antibody (mAb) formulations. The method consists of measuring the fluorescence intensity of a polarity sensitive probe at gradually increasing temperatures, and obtaining th