The composition of fatty acids of human adipose tissue reflects the composition of fatty acids in diet [5, 7, 8, 10, 1 I]. It also depends to different extent on such factors as age [I 1, 131, sex [4, 1 I, 131, race [4, 13, 141, physical activity [18], physical condition (heart diseases) [14, 191 or adipose tissue kind [13].
Lack of data concerning the composition of fatty acids in adipose tissue of Poles made us undertake such studies. As there are no literature data available on triglyceride composition of human adipose! tissue we decided to include this subject in our investigations.
Material and methods
Samples of perirenal adipose tissue were collected during anatomopathological examination at Medical
Academy in Bialystok (North-East Poland). There were analysed 32 samples -18 from men, 14 from women; 10 from people aged between 41-60,22 from people aged between 61-91.
Fat was extracted from tissue according to Folch et al. [9]. Fatty acid composition after methylation according to Peisker [I61 was determined with GLC in a gas chromatograph Pye Unicam 104 with FID joined with a recorder (Philips) and integrator Pye Unicam DP-88. Separation conditions: glass column 2.1 m long, inside diameter 4 mm with 10 X DEGS + 20 X H3PO4 on Varaport 30 100-200 mesh. Temperature of column 195 C, of detector 250 C, of evaporator 225 C.
To analyse the composition of triglycerides fat dissolved in ethyl ether (5 46 solution) was injected into the column.
Separation conditions: glass column 0.6 m long, inside diameter 4 mm with 3 X OV-17 on Chromosorb