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Complexes of Cationic Block Copolymer Micelles with DNA: Histone/DNA Complex Mimetics

✍ Scribed by Marina Talelli; Stergios Pispas

Publisher: John Wiley and Sons
Year: 2008
Tongue: English
Weight: 704 KB
Volume: 8
Category: Article
ISSN: 1616-5187
DOI: 10.1002/mabi.200800075

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✦ Synopsis

Abstract

The formation of complexes between cationic polymeric micelles of PS‐b‐PQ2VP amphiphilic block copolymers and DNA molecules in aqueous solutions is investigated at pH = 7. The physicochemical characteristics of the “polyplexes” at different DNA/polymer ratios were characterized in terms of mass, size and charge using static, dynamic and electrophoretic light scattering and AFM. The complexes are spherical and assume their maximum size and mass around the charge stoichiometric ratio. After addition of increased amounts of salt in the solutions, partial dissociation of the systems was observed. The present systems can be considered as mimetics of histone/DNA complexes formed under physiological conditions in living cells.

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