Complete separation of the geometical isomers of linolenic acid by high performance liquid chromatography with a silver ion column

✍ Scribed by Juanéda, Pierre ;Sebedio, Jean Louis ;Christie, William W.

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 349 KB
• Volume: 17
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240170508

✦ Synopsis

Abstract

A method has been developed for separation of linolenic acid and its seven isomers by HPLC on a silver‐ion‐loaded column. The standard 18:3 isomers, isolated from a heated linseed oil or prepared by isomerization of linolenic acid, were converted into phenacyl esters and detected by UV at 238 nm. The use of low temperature (10 °C) combined with a gradient of dichloromethane and methanol enabled separation of all the cis/trans isomers. The peaks were identified by comparison of ECL values with those of a standard mixture, by chromatographing collected HPLC fractions on a polar GC column. HPLC quantification was compared with GC analysis. There was satisfactory agreement between the tow methods. This method could be used for seperation, collection and quantification of 18:3 fatty acids with trans double bonds in different oils and foods.