✦ LIBER ✦

Complete replication of human cytomegalovirus in explants of first trimester human placenta

✍ Scribed by Liliana Gabrielli; Luisa Losi; Stefania Varani; Tiziana Lazzarotto; Vincenzo Eusebi; Maria Paola Landini

Publisher: John Wiley and Sons
Year: 2001
Tongue: English
Weight: 350 KB
Volume: 64
Category: Article
ISSN: 0146-6615
DOI: 10.1002/jmv.1077

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Tissue integrity and viability of first trimester placenta explants were obtained in culture for 3 weeks. Explants were infected with human cytomegalovirus (HCMV), several cycles of HCMV replication were obtained and the progression of the infection was observed within a tissue that maintains its normal cellular organization. In agreement with recent clinical data, 3 weeks were necessary for the virus to colonize the placenta fully. Complete HCMV replication was observed in trophoblasts, followed by subsequent transmission of the infection to the stromal fibroblasts and fetal endothelial capillary cells. Viral DNA replication was monitored and the production of infectious viral progeny documented. J. Med. Virol. 64:499–504, 2001. © 2001 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Detection of cytomegalovirus DNA in huma

Detection of cytomegalovirus DNA in human placenta

✍ Kaori Kumazaki; Keiichi Ozono; Takeshi Yahara; Yoshinao Wada; Noriyuki Suehara; 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 306 KB

## Abstract Although human cytomegalovirus (CMV) is one of the most common causes of viral intrauterine and perinatal infection, its distribution in the placenta is poorly understood. The purpose of this study was to determine the frequency of CMV DNA positivity in placentas, to demonstrate the loc

High α-amylase activity in the syncytiot

High α-amylase activity in the syncytiotrophoblastic cells of first-trimester human placentas

✍ Susan J. Fisher; Roger A. Laine 📂 Article 📅 1983 🏛 John Wiley and Sons 🌐 English ⚖ 569 KB

The syncytiotrophoblastic brush border of the human placenta forms the maternalfetal interface and is an important determinant of placental function. Electron micrographs of fresh brush border preparations isolated from first-trimester human placentas showed membrane vesicles, open-ended microvilli,

Degradation of extracellular matrix by t

Degradation of extracellular matrix by the trophoblastic cells of first-trimester human placentas

✍ Susan J. Fisher; Mark S. Leitch; Marsha S. Kantor; Carol B. Basbaum; Randall H. 📂 Article 📅 1985 🏛 John Wiley and Sons 🌐 English ⚖ 917 KB

First-trimester human placental villi were cultured on 3H-leucine-labeled extracellular matrices isolated from the PF HR9 and PYS-2 cell lines. Both cell lines produced an extracellular matrix that contained basement membrane-specific macromolecules, including type IV collagen, laminin and proteogly

Distinct expression and localization of

Distinct expression and localization of serine protease HtrA1 in human endometrium and first-trimester placenta

✍ Guiying Nie; Kathryn Hale; Ying Li; Ursula Manuelpillai; Euan M. Wallace; Lois A 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 472 KB 👁 1 views

Monoclonal antibody to human T-cell leuk

Monoclonal antibody to human T-cell leukemia virus P19 defines polypeptide antigen in human choriocarcinoma cells and syncytiotrophoblasts of first-trimester placentas

✍ Dr. Jukka Suni; Ale Närvänen; Torsten Wahlström; Pentti Lehtovirta; Antti Vaheri 📂 Article 📅 1984 🏛 John Wiley and Sons 🌐 French ⚖ 781 KB

## Abstract In previous studies we detected retrovirus RD114 p30‐related antigen in human placental syncytiotrophoblasts and antibodies in cord blood sera. We now report that a monoclonal IgG antibody specific for the p19 protein of human T‐cell leukemia virus (HTLV) reacts with human syncytiotroph

Restriction to human cytomegalovirus rep

Restriction to human cytomegalovirus replication in vitro removed by physiological levels of cortisol

✍ Dr. Roger W. Koment 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 381 KB

Since viral infection is in most cases contrary to the survival of the host cell, it is reasonable to assume that cells possess innate viral replication inhibitory mechanisms. Even between strains of permissive cells, degrees of permissiveness are observed. Restriction to human cytomegalovir u s (CM