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Complementation of a dnaC initiation defect in vitro

✍ Scribed by Projan, Steven J. ;Wechsler, James A.

Publisher
Springer
Year
1981
Tongue
English
Weight
395 KB
Volume
183
Category
Article
ISSN
0026-8925

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✦ Synopsis

The dnaC28 mutant, CT28-3b, is an initiation defective dnaC strain. Extracts of the mutant failed to synthesize DNA in vitro when the strain was incubated at the restrictive temperature for two generation times prior to preparation of the extract. Addition of a complementing extract from a Col-E1::dnaC+ hybrid plasmid containing strain or of partially purified dnaC protein resulted in substantial synthesis. Hybridization of the DNA made by these in vitro complementation extracts showed that a significant portion of this DNA was from the region near the chromosomal origin of replication.

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